A Chromosome-Scale Assembly of the Garden Orach (Atriplex hortensis L.) Genome Using Oxford Nanopore Sequencing
نویسندگان
چکیده
منابع مشابه
Oxford Nanopore MinION Sequencing and Genome Assembly
The revolution of genome sequencing is continuing after the successful second-generation sequencing (SGS) technology. The third-generation sequencing (TGS) technology, led by Pacific Biosciences (PacBio), is progressing rapidly, moving from a technology once only capable of providing data for small genome analysis, or for performing targeted screening, to one that promises high quality de novo ...
متن کاملEffect of Seed Weight and Salinity on the Germination of Garden Orache (Atriplex hortensis L.)
Garden orache (Atriplex hortensis L.), a halophytic forb in the family Chenopodiaceae, is well adapted to dry saline habitats. Here, we presented the results of the effect of salinity and seed weight on seed germination and radicle emergence of A. hortensis. Highest germination percentages were obtained under non-saline conditions and increases in salinity inhibited seed germination with less t...
متن کاملOxford Nanopore sequencing, hybrid error correction, and de novo assembly of a eukaryotic genome.
Monitoring the progress of DNA molecules through a membrane pore has been postulated as a method for sequencing DNA for several decades. Recently, a nanopore-based sequencing instrument, the Oxford Nanopore MinION, has become available, and we used this for sequencing the Saccharomyces cerevisiae genome. To make use of these data, we developed a novel open-source hybrid error correction algorit...
متن کاملOxford Nanopore Sequencing and de novo Assembly of a Eukaryotic Genome
Monitoring the progress of DNA through a pore has been postulated as a method for sequencing DNA for several decades 1,2. Recently, a nanopore instrument, the Oxford Nanopore MinION, has become available 3. Here we describe our sequencing of the S. cerevisiae genome. We describe software developed to make use of these data as existing packages were incapable of assembling long reads at such hig...
متن کاملChallenges in Transcriptome Applications Using Oxford Nanopore Sequencing Technology
1School of Biological Sciences, Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln NE 68588, USA 2Department of Computer Science and Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA *Corresponding author: Kan Liu, School of Biological Sciences, Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln NE 68588, USA, Tel: +1 402-472...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Frontiers in Plant Science
سال: 2020
ISSN: 1664-462X
DOI: 10.3389/fpls.2020.00624